Biodegradation of phenol by alkaliphiic Bacillus badius D1

Phenols have been recognized as mutagenic and carcinogenic. Industrial effluents possessing phenols causes’ environmental pollution. Present study was planned to investigate the % degradation of phenol using alkaliphilic Bacillus badius D1 with various concentration ranging from 0.42 to 1.68 g/L in alkaline broth of pH-9, in shaking incubator at110 rpm, 37 0 C. The % degradation at lowest concentration was about 98%. Even at higher concentration the rate of degradation was found higher than 70 %. The % degradation and spectroscopic profile was monitored from 0 to 48 hr. by 6 hr. interval. The metabolic pathway was constructed by isolating and characterizing the metabolites by 1 HNMR, FTIR, and GCMS. It was concluded that the Bacillus badius follows ortho and meta catechol pathway for degradation. The biodegradation study was extended for varying parameters like temperature, pH, salinity, additional carbon and nitrogen sources.